Biology Microscopy
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- Created by: RhiannonAugust
- Created on: 12-09-17 11:34
How do you prepare a dry mount sample?
1. Sample. 2. Put on Slide. 3. Cover Slip. 4.Sample Prepared.
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How do you prepare a wet mount sample?
1. Sample. 2. Add moisture (oil or water). 3. Cover Slip. 4. Sample Prepared.
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How do you prepare a squash slide sample?
1. Prepare wet mount. 2. Add lens tissue on top. 3. Gently push a glass slide on top to squash the sample.
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How do you prepare smear slides?
1. Put sample on slide. 2. Smear the sample to separate it. 3. You will see more cells.
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What would you sample on a wet mount?
Bacteria. As it can extend the lifespan of some bacterias.
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What would you sample on a smear slide?
Blood or slime.
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What would you use squash slides to sample?
Soft samples.
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Name two positively charged dyes.
1. Crystal violet. 2. Methylene blue.
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Why use positively charged dyes?
They are attracted to negatively charged materials in cytoplasm.
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Name two negatively charged dyes.
1. Congo red. 2. Nigrosin.
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Why use negatively charged dyes?
They are repelled from the negatively charged cytosol and stay outside of the cells. Therefore the cells stand out against the stained background.
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What is acid fast staining used for?
Used for materials that resist different staining methods. And differentiate different species of microbacterium.
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How do you carry out acid fast staining?
1. A lipid solvent is used to carry dye into cells. 2. Cells washed with a dilute acid-alcohol solution. 3. Mycrobacterium arent affected by acid solution and retain there colour and other bacteria lose their stain.
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Suggest a good stain to use to investigate onion cells?
Iodine.
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Why do samples need to be stained?
The cell structure is see through.
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What is the definition of magnification?
How many times larger the image is than the original image.
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What word equation would you use to work out the actual size of an image?
Actual Size= Image Size/Magnification
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What word equation would you use to work out the magnification of an image?
Magnification= Image Size/Actual Size
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By the 1950s biologists had developed which two microscopes?
1. Electron Microscope. 2. Laser Scanning Confocal Microscope.
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What are the two types of electron microscopes?
1. Transmission electron microscope (TEM). 2. Scanning electron microscopes (SEM)
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What are the advantages of using a TEM?
1. Electrons pass through. 2. The magnification is x500,000
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What are the disadvantages of using a TEM?
1. Only produces 2D images.
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What are the advantages of using a SEM?
1. Higher resolution than a light microscope. 2. Images are 3D.
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What are the disadvantage of using a SEM?
1. Electrons reflect off. 2. Magnification is only x100,000, which is lower than a TEM.
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What is the maximum magnification that can be achieved by a light microscope?
x1500.
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What is the definition of the term resolution?
The distinguishing of two objects in close proximity and shows us more detail.
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When and who first observed cells?
In 1665 Rober Hooke observed the cells of a cork and described the compartments as cells. But only saw cell walls as the plants cells were dead.
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When and who first observed a living cell (Bacteria and Protoctista)?
In 1674-1683 Anton Van Leeuwenhoek developed powerful glass lenses and examined pond water. He observed red blood cells, sperm cells and muscle tissue.
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When and who observed cell division?
In 1832 Barthelemy Dumortier observed cell division and people disagreed with him because of conflicting theory.
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When and who observed the first nucleus?
In 1833 Robert Brown was the first to describe the nucleus of a plant cell.
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What is the Universal Cell Theory?
Not only animals are made out of cells. 'basic cellular tissue is clearly anagulous to that of plants'
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Who and when came up with the Universal Cell Theory?
In 1837-1838 Matthias proposed the universal cell theory. Theodor Schwann made a similar observation 'all living things are composed of cells'.'
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When and who first observed cell division in animal cells?
In 1844 (1855) Robert Remak observed cell division in animal cells which disproved other theories. But this wasn't accepted by fellow scientists.
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When and who disproved spontaneous generation?
In 1860 Louis Pasteur disproved spontaneous generation.
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Other cards in this set
Card 2
Front
How do you prepare a wet mount sample?
Back
1. Sample. 2. Add moisture (oil or water). 3. Cover Slip. 4. Sample Prepared.
Card 3
Front
How do you prepare a squash slide sample?
Back
Card 4
Front
How do you prepare smear slides?
Back
Card 5
Front
What would you sample on a wet mount?
Back
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