(4) Gene Transfer

Technique for gene transfer

Gene transfer involves the following elements: 

- vector to carry the gene into the host cell. (plasmids are very common) 

- host cell

- restriction enzymes

- DNA ligase

Bacteria carry al required genetic info on one large circular DNA. They also possess extra DNA in the form of plasmids. 

1. spice the desired gene into a plasmid and transfer it into a bacterial cell. 

2. Culture bacteria, many will have have a plasmid with the desire gene. 

3. Use a section of nucelotides completementary to the desired genes but also…