Root Tip Squash - Core Practical
- Created by: hope rowden
- Created on: 02-06-13 14:04
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- Root Tip Squash
- Experimental Procedure
- growing garlic tip, cut off 10mm
- Soak in HCl - break down cell walls
- wash with distilled water - get rid of acid
- place 2mm of rounded end of root on to glass slide
- stain in
- macerate it - tip of needle or end of tweezers
- cover with cover slide, gently squash
- view under microscope
- cover with cover slide, gently squash
- macerate it - tip of needle or end of tweezers
- stain in
- place 2mm of rounded end of root on to glass slide
- wash with distilled water - get rid of acid
- Soak in HCl - break down cell walls
- growing garlic tip, cut off 10mm
- Aim
- To demonstrate MITOSIS in growing roots
- Safety considerations
- stain could get into eyes
- wear googles
- knife could cut you
- always cut away from yourself
- cut on tile
- stain could get onto clothes
- wear a lab coat
- stain could get into eyes
- Chemicals or anything else
- Aretic Orcein
- Toluidine blue
- Experimental Procedure
- view under microscope
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