Chromatography
Mind map on chromatography :)
- Created by: Jessica Scott
- Created on: 23-04-13 13:29
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- Chromotography
- Adsorption Chromatography
- Stationary phase is solid e.g Alumina
- Moving phase is lquid or gas
- Adsorption Chromatography
- Chromotogrpahy is a technique for seperating the compounds of a mixture on the basis of differences in their affinity for a stationary phase and a moving phase
- Partition Chromatography
- Stationary phase is a non volatile liquid held on a surface inert
- Chromotography
- Adsorption Chromatography
- Stationary phase is solid e.g Alumina
- Moving phase is lquid or gas
- Adsorption Chromatography
- Seperation depends on the balance between solute solubility in the moving phase and retention in the stationary phase
- Partition Chromatography
- Stationary phase is a non volatile liquid held on a surface inert
- Partition Chromatography
- Paper (Partition Chromatogrpahy)
- Stationary phase is water adsorbed onto cellulose fibres of paper
- Spots visible by UV light
- Thin layer Chromatography
- Concentrated solution is applied at base rises by capillary action
- Advantages: quick, efficent, reproducible
- Thin layer Chromatography
- Adsorption Chromatography
- Column Chromatography
- Alumina or Silica gel as stationary phase
- most strongly absorbed take the longest to flow through the column, the most polar
- Speed increases by increase pressure
- Column Chromatography
- Then seperatedin batches and analysed
- most strongly absorbed take the longest to flow through the column, the most polar
- Speed increases by increase pressure
- most strongly absorbed take the longest to flow through the column, the most polar
- A detector lined to a recorder produces a chromatogram
- Each will have a retention time therefore can be compared with known samples
- Mass spectrometer can be used
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